Method Statement BOD
@(#) Uncontrolled Document bod.html Version 1.1 Last
Revised on 12/09/02
Contents
Introduction
Principle
Performance
Characteristics
Range
of Application
Limit of
Detection
Analytical
Quality Control
References
Introduction
The performance of this method is validated in accordance with
internationally recognised procedures.
This procedure describes the determination of biological oxygen
demand (BOD) in waters by incubation and determination of dissolved
oxygen by means of a dissolved oxygen meter.
Principle
BOD (5 day) is determined by dilution of an aliquot of
air-saturated sample and, if appropriate, "seeding" with a
suitable source of micro-organisms. The container is then incubated
for five days at 20 degrees Centigrade in the dark. The amount of
oxygen absorbed is determined by means of a dissolved oxygen meter.
Performance
Characteristics
Substances Determined
Uptake of dissolved oxygen by the sample during 5 days at 20
degrees centigrade in the dark.
Range of Application
Limit of Detection
Analytical Quality
Control
Analytical quality control is maintained by a number of measures:
Multi-point calibration with
authentic standards (with defined minimum performance
characteristics)
Analysis of control samples within
each analytical batch, such as independent standards, matrix spikes
or reference materials.
Participation in external
proficiency testing and interlaboratory schemes such as LGC CONTEST,
HSE WASP, CSL FAPAS
References
5 Day Biochemical Oxygen
Demand (BOD5) in Waters, DOE Methods for the Examination of Waters
and Associated Materials, published by HMSO. 1988.
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